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m-per mammalian protein extraction reagent  (Thermo Fisher)


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    Thermo Fisher m-per mammalian protein extraction reagent
    M Per Mammalian Protein Extraction Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/m-per+mammalian+protein+extraction+reagent/m+per+mammalian+protein+extraction+reagent/pmc12221594-147-6-12
    Average 90 stars, based on 1 article reviews
    m-per mammalian protein extraction reagent - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Protein Extraction:

    Article Title: miRNA-29b-1-5p mediates an antiviral activity by targeting the HBV entry receptor in human hepatocytes.
    Article Snippet: Cells were collected 24 h posttransfection, and protein was extracted using the M-PER mammalian protein extraction reagent (ThermoFisher Scientific).

    Article Title: Suppression of multiple mouse models of refractory malignancies by reprogramming IL-18 ligand-receptor interaction.
    Article Snippet: Cells were lysed by M-PER Mammalian Protein Extraction Reagent (ThermoScientific).

    Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27.
    Article Snippet: Cells were lysed using M-PERTM Mammalian Protein Extraction Reagent (Thermo Fisher Scientific cat. no. 78501) lysis buffer, supplemented with HaltTM Protease and Phosphatase Inhibitor Single-Use Cocktail (100x) (Thermo Fisher Scientific cat. no. 78442).

    Article Title: Loss of serine/threonine protein phosphatase 6 severely impairs sexual stage development in malaria parasite Plasmodium berghei.
    Article Snippet: Proteins were extracted using M-PER Mammalian Protein Extraction Reagent (Thermo Fisher) and quantified with the Pierce BCA Protein Assay Kit (Thermo Fisher), followed by resolution via 10% SDS-PAGE.

    Article Title: Insulin resistance, cognition, and functional brain network topology in older adults with obesity.
    Article Snippet: Each suspension then received 450ul M-PER mammalian protein extraction reagent (Thermo Fisher Scientific, Incorporated) that had been adjusted to pH 8 with 1 M Tris HCL (pH 8.6) and contained the cocktails of protease and phosphatase inhibitors.

    Article Title: Human induced pluripotent stem cell derived nanovesicles for cardiomyocyte protection and proliferation
    Article Snippet: It remains a significant challenge to reactivate the cell cycle activity of adult mammalian cardiomyocytes (CMs).. This study created a hypo-immunogenic human induced pluripotent stem cell (hiPSC) line using clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 gene editing to knockout β2-microglobulin in hiPSCs (B2MKOhiPSCs) for manufacturing nanovesicles (B2MKOhiPSC-NVs).. Approximately 9500 B2MKOhiPSC-NVs were produced from a single B2MKOhiPSC.

    Article Title: Exogenous pyruvate is therapeutic against colitis by targeting cytosolic phospholipase A2
    Article Snippet: Briefly, RAW264.7 cells were lysed using M-PERTM Mammalian Protein Extraction Reagent (78501, Thermofisher), followed by centrifugation at 18,000 g and 4 °C for 10 min.

    Article Title: Diammonium Glycyrrhizinate Exerts Broad-Spectrum Antiviral Activity Against Human Coronaviruses by Interrupting Spike-Mediated Cellular Entry.
    Article Snippet: Cellular proteins were extracted from cell lysates using M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific, New York, NY, USA) containing halt protease inhibitor single-use cocktail and then denatured in protein loading buffer at 100 ◦C for 10 min. Immunoblotting for HCoV-OC43 nucleocapsid protein (Millipore, Billerica, MA, USA, 1:1000) and HCoV-229E nucleocapsid protein (Sino Biological, Beijing, China, 1:1000) was performed as described previously [40].

    Centrifugation:

    Article Title: miRNA-29b-1-5p mediates an antiviral activity by targeting the HBV entry receptor in human hepatocytes.
    Article Snippet: Cells were collected 24 h posttransfection, and protein was extracted using the M-PER mammalian protein extraction reagent (ThermoFisher Scientific).

    Article Title: Suppression of multiple mouse models of refractory malignancies by reprogramming IL-18 ligand-receptor interaction.
    Article Snippet: Cells were lysed by M-PER Mammalian Protein Extraction Reagent (ThermoScientific).

    Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27.
    Article Snippet: Cells were lysed using M-PERTM Mammalian Protein Extraction Reagent (Thermo Fisher Scientific cat. no. 78501) lysis buffer, supplemented with HaltTM Protease and Phosphatase Inhibitor Single-Use Cocktail (100x) (Thermo Fisher Scientific cat. no. 78442).

    Article Title: Loss of serine/threonine protein phosphatase 6 severely impairs sexual stage development in malaria parasite Plasmodium berghei.
    Article Snippet: Proteins were extracted using M-PER Mammalian Protein Extraction Reagent (Thermo Fisher) and quantified with the Pierce BCA Protein Assay Kit (Thermo Fisher), followed by resolution via 10% SDS-PAGE.

    Article Title: Insulin resistance, cognition, and functional brain network topology in older adults with obesity.
    Article Snippet: Each suspension then received 450ul M-PER mammalian protein extraction reagent (Thermo Fisher Scientific, Incorporated) that had been adjusted to pH 8 with 1 M Tris HCL (pH 8.6) and contained the cocktails of protease and phosphatase inhibitors.

    Article Title: Human induced pluripotent stem cell derived nanovesicles for cardiomyocyte protection and proliferation
    Article Snippet: It remains a significant challenge to reactivate the cell cycle activity of adult mammalian cardiomyocytes (CMs).. This study created a hypo-immunogenic human induced pluripotent stem cell (hiPSC) line using clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 gene editing to knockout β2-microglobulin in hiPSCs (B2MKOhiPSCs) for manufacturing nanovesicles (B2MKOhiPSC-NVs).. Approximately 9500 B2MKOhiPSC-NVs were produced from a single B2MKOhiPSC.

    Article Title: Exogenous pyruvate is therapeutic against colitis by targeting cytosolic phospholipase A2
    Article Snippet: Briefly, RAW264.7 cells were lysed using M-PERTM Mammalian Protein Extraction Reagent (78501, Thermofisher), followed by centrifugation at 18,000 g and 4 °C for 10 min.

    Article Title: Diammonium Glycyrrhizinate Exerts Broad-Spectrum Antiviral Activity Against Human Coronaviruses by Interrupting Spike-Mediated Cellular Entry.
    Article Snippet: Cellular proteins were extracted from cell lysates using M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific, New York, NY, USA) containing halt protease inhibitor single-use cocktail and then denatured in protein loading buffer at 100 ◦C for 10 min. Immunoblotting for HCoV-OC43 nucleocapsid protein (Millipore, Billerica, MA, USA, 1:1000) and HCoV-229E nucleocapsid protein (Sino Biological, Beijing, China, 1:1000) was performed as described previously [40].

    Western Blot:

    Article Title: miRNA-29b-1-5p mediates an antiviral activity by targeting the HBV entry receptor in human hepatocytes.
    Article Snippet: Cells were collected 24 h posttransfection, and protein was extracted using the M-PER mammalian protein extraction reagent (ThermoFisher Scientific).

    Article Title: Suppression of multiple mouse models of refractory malignancies by reprogramming IL-18 ligand-receptor interaction.
    Article Snippet: Cells were lysed by M-PER Mammalian Protein Extraction Reagent (ThermoScientific).

    Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27.
    Article Snippet: Cells were lysed using M-PERTM Mammalian Protein Extraction Reagent (Thermo Fisher Scientific cat. no. 78501) lysis buffer, supplemented with HaltTM Protease and Phosphatase Inhibitor Single-Use Cocktail (100x) (Thermo Fisher Scientific cat. no. 78442).

    Article Title: Loss of serine/threonine protein phosphatase 6 severely impairs sexual stage development in malaria parasite Plasmodium berghei.
    Article Snippet: Proteins were extracted using M-PER Mammalian Protein Extraction Reagent (Thermo Fisher) and quantified with the Pierce BCA Protein Assay Kit (Thermo Fisher), followed by resolution via 10% SDS-PAGE.

    Article Title: Insulin resistance, cognition, and functional brain network topology in older adults with obesity.
    Article Snippet: Each suspension then received 450ul M-PER mammalian protein extraction reagent (Thermo Fisher Scientific, Incorporated) that had been adjusted to pH 8 with 1 M Tris HCL (pH 8.6) and contained the cocktails of protease and phosphatase inhibitors.

    Article Title: Human induced pluripotent stem cell derived nanovesicles for cardiomyocyte protection and proliferation
    Article Snippet: It remains a significant challenge to reactivate the cell cycle activity of adult mammalian cardiomyocytes (CMs).. This study created a hypo-immunogenic human induced pluripotent stem cell (hiPSC) line using clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 gene editing to knockout β2-microglobulin in hiPSCs (B2MKOhiPSCs) for manufacturing nanovesicles (B2MKOhiPSC-NVs).. Approximately 9500 B2MKOhiPSC-NVs were produced from a single B2MKOhiPSC.

    Article Title: Exogenous pyruvate is therapeutic against colitis by targeting cytosolic phospholipase A2
    Article Snippet: Briefly, RAW264.7 cells were lysed using M-PERTM Mammalian Protein Extraction Reagent (78501, Thermofisher), followed by centrifugation at 18,000 g and 4 °C for 10 min.

    Article Title: Diammonium Glycyrrhizinate Exerts Broad-Spectrum Antiviral Activity Against Human Coronaviruses by Interrupting Spike-Mediated Cellular Entry.
    Article Snippet: Cellular proteins were extracted from cell lysates using M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific, New York, NY, USA) containing halt protease inhibitor single-use cocktail and then denatured in protein loading buffer at 100 ◦C for 10 min. Immunoblotting for HCoV-OC43 nucleocapsid protein (Millipore, Billerica, MA, USA, 1:1000) and HCoV-229E nucleocapsid protein (Sino Biological, Beijing, China, 1:1000) was performed as described previously [40].

    Protein-Protein interactions:

    Article Title: miRNA-29b-1-5p mediates an antiviral activity by targeting the HBV entry receptor in human hepatocytes.
    Article Snippet: Cells were collected 24 h posttransfection, and protein was extracted using the M-PER mammalian protein extraction reagent (ThermoFisher Scientific).

    Article Title: Suppression of multiple mouse models of refractory malignancies by reprogramming IL-18 ligand-receptor interaction.
    Article Snippet: Cells were lysed by M-PER Mammalian Protein Extraction Reagent (ThermoScientific).

    Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27.
    Article Snippet: Cells were lysed using M-PERTM Mammalian Protein Extraction Reagent (Thermo Fisher Scientific cat. no. 78501) lysis buffer, supplemented with HaltTM Protease and Phosphatase Inhibitor Single-Use Cocktail (100x) (Thermo Fisher Scientific cat. no. 78442).

    Article Title: Loss of serine/threonine protein phosphatase 6 severely impairs sexual stage development in malaria parasite Plasmodium berghei.
    Article Snippet: Proteins were extracted using M-PER Mammalian Protein Extraction Reagent (Thermo Fisher) and quantified with the Pierce BCA Protein Assay Kit (Thermo Fisher), followed by resolution via 10% SDS-PAGE.

    Article Title: Insulin resistance, cognition, and functional brain network topology in older adults with obesity.
    Article Snippet: Each suspension then received 450ul M-PER mammalian protein extraction reagent (Thermo Fisher Scientific, Incorporated) that had been adjusted to pH 8 with 1 M Tris HCL (pH 8.6) and contained the cocktails of protease and phosphatase inhibitors.

    Article Title: Human induced pluripotent stem cell derived nanovesicles for cardiomyocyte protection and proliferation
    Article Snippet: It remains a significant challenge to reactivate the cell cycle activity of adult mammalian cardiomyocytes (CMs).. This study created a hypo-immunogenic human induced pluripotent stem cell (hiPSC) line using clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 gene editing to knockout β2-microglobulin in hiPSCs (B2MKOhiPSCs) for manufacturing nanovesicles (B2MKOhiPSC-NVs).. Approximately 9500 B2MKOhiPSC-NVs were produced from a single B2MKOhiPSC.

    Article Title: Exogenous pyruvate is therapeutic against colitis by targeting cytosolic phospholipase A2
    Article Snippet: Briefly, RAW264.7 cells were lysed using M-PERTM Mammalian Protein Extraction Reagent (78501, Thermofisher), followed by centrifugation at 18,000 g and 4 °C for 10 min.

    Article Title: Diammonium Glycyrrhizinate Exerts Broad-Spectrum Antiviral Activity Against Human Coronaviruses by Interrupting Spike-Mediated Cellular Entry.
    Article Snippet: Cellular proteins were extracted from cell lysates using M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific, New York, NY, USA) containing halt protease inhibitor single-use cocktail and then denatured in protein loading buffer at 100 ◦C for 10 min. Immunoblotting for HCoV-OC43 nucleocapsid protein (Millipore, Billerica, MA, USA, 1:1000) and HCoV-229E nucleocapsid protein (Sino Biological, Beijing, China, 1:1000) was performed as described previously [40].

    Isolation:

    Article Title: miRNA-29b-1-5p mediates an antiviral activity by targeting the HBV entry receptor in human hepatocytes.
    Article Snippet: Cells were collected 24 h posttransfection, and protein was extracted using the M-PER mammalian protein extraction reagent (ThermoFisher Scientific).

    Article Title: Suppression of multiple mouse models of refractory malignancies by reprogramming IL-18 ligand-receptor interaction.
    Article Snippet: Cells were lysed by M-PER Mammalian Protein Extraction Reagent (ThermoScientific).

    Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27.
    Article Snippet: Cells were lysed using M-PERTM Mammalian Protein Extraction Reagent (Thermo Fisher Scientific cat. no. 78501) lysis buffer, supplemented with HaltTM Protease and Phosphatase Inhibitor Single-Use Cocktail (100x) (Thermo Fisher Scientific cat. no. 78442).

    Article Title: Loss of serine/threonine protein phosphatase 6 severely impairs sexual stage development in malaria parasite Plasmodium berghei.
    Article Snippet: Proteins were extracted using M-PER Mammalian Protein Extraction Reagent (Thermo Fisher) and quantified with the Pierce BCA Protein Assay Kit (Thermo Fisher), followed by resolution via 10% SDS-PAGE.

    Article Title: Insulin resistance, cognition, and functional brain network topology in older adults with obesity.
    Article Snippet: Each suspension then received 450ul M-PER mammalian protein extraction reagent (Thermo Fisher Scientific, Incorporated) that had been adjusted to pH 8 with 1 M Tris HCL (pH 8.6) and contained the cocktails of protease and phosphatase inhibitors.

    Article Title: Human induced pluripotent stem cell derived nanovesicles for cardiomyocyte protection and proliferation
    Article Snippet: It remains a significant challenge to reactivate the cell cycle activity of adult mammalian cardiomyocytes (CMs).. This study created a hypo-immunogenic human induced pluripotent stem cell (hiPSC) line using clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 gene editing to knockout β2-microglobulin in hiPSCs (B2MKOhiPSCs) for manufacturing nanovesicles (B2MKOhiPSC-NVs).. Approximately 9500 B2MKOhiPSC-NVs were produced from a single B2MKOhiPSC.

    Article Title: Exogenous pyruvate is therapeutic against colitis by targeting cytosolic phospholipase A2
    Article Snippet: Briefly, RAW264.7 cells were lysed using M-PERTM Mammalian Protein Extraction Reagent (78501, Thermofisher), followed by centrifugation at 18,000 g and 4 °C for 10 min.

    Article Title: Diammonium Glycyrrhizinate Exerts Broad-Spectrum Antiviral Activity Against Human Coronaviruses by Interrupting Spike-Mediated Cellular Entry.
    Article Snippet: Cellular proteins were extracted from cell lysates using M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific, New York, NY, USA) containing halt protease inhibitor single-use cocktail and then denatured in protein loading buffer at 100 ◦C for 10 min. Immunoblotting for HCoV-OC43 nucleocapsid protein (Millipore, Billerica, MA, USA, 1:1000) and HCoV-229E nucleocapsid protein (Sino Biological, Beijing, China, 1:1000) was performed as described previously [40].

    Protease Inhibitor:

    Article Title: miRNA-29b-1-5p mediates an antiviral activity by targeting the HBV entry receptor in human hepatocytes.
    Article Snippet: Cells were collected 24 h posttransfection, and protein was extracted using the M-PER mammalian protein extraction reagent (ThermoFisher Scientific).

    Article Title: Suppression of multiple mouse models of refractory malignancies by reprogramming IL-18 ligand-receptor interaction.
    Article Snippet: Cells were lysed by M-PER Mammalian Protein Extraction Reagent (ThermoScientific).

    Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27.
    Article Snippet: Cells were lysed using M-PERTM Mammalian Protein Extraction Reagent (Thermo Fisher Scientific cat. no. 78501) lysis buffer, supplemented with HaltTM Protease and Phosphatase Inhibitor Single-Use Cocktail (100x) (Thermo Fisher Scientific cat. no. 78442).

    Article Title: Loss of serine/threonine protein phosphatase 6 severely impairs sexual stage development in malaria parasite Plasmodium berghei.
    Article Snippet: Proteins were extracted using M-PER Mammalian Protein Extraction Reagent (Thermo Fisher) and quantified with the Pierce BCA Protein Assay Kit (Thermo Fisher), followed by resolution via 10% SDS-PAGE.

    Article Title: Insulin resistance, cognition, and functional brain network topology in older adults with obesity.
    Article Snippet: Each suspension then received 450ul M-PER mammalian protein extraction reagent (Thermo Fisher Scientific, Incorporated) that had been adjusted to pH 8 with 1 M Tris HCL (pH 8.6) and contained the cocktails of protease and phosphatase inhibitors.

    Article Title: Human induced pluripotent stem cell derived nanovesicles for cardiomyocyte protection and proliferation
    Article Snippet: It remains a significant challenge to reactivate the cell cycle activity of adult mammalian cardiomyocytes (CMs).. This study created a hypo-immunogenic human induced pluripotent stem cell (hiPSC) line using clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 gene editing to knockout β2-microglobulin in hiPSCs (B2MKOhiPSCs) for manufacturing nanovesicles (B2MKOhiPSC-NVs).. Approximately 9500 B2MKOhiPSC-NVs were produced from a single B2MKOhiPSC.

    Article Title: Exogenous pyruvate is therapeutic against colitis by targeting cytosolic phospholipase A2
    Article Snippet: Briefly, RAW264.7 cells were lysed using M-PERTM Mammalian Protein Extraction Reagent (78501, Thermofisher), followed by centrifugation at 18,000 g and 4 °C for 10 min.

    Article Title: Diammonium Glycyrrhizinate Exerts Broad-Spectrum Antiviral Activity Against Human Coronaviruses by Interrupting Spike-Mediated Cellular Entry.
    Article Snippet: Cellular proteins were extracted from cell lysates using M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific, New York, NY, USA) containing halt protease inhibitor single-use cocktail and then denatured in protein loading buffer at 100 ◦C for 10 min. Immunoblotting for HCoV-OC43 nucleocapsid protein (Millipore, Billerica, MA, USA, 1:1000) and HCoV-229E nucleocapsid protein (Sino Biological, Beijing, China, 1:1000) was performed as described previously [40].

    Bicinchoninic Acid Protein Assay:

    Article Title: miRNA-29b-1-5p mediates an antiviral activity by targeting the HBV entry receptor in human hepatocytes.
    Article Snippet: Cells were collected 24 h posttransfection, and protein was extracted using the M-PER mammalian protein extraction reagent (ThermoFisher Scientific).

    Article Title: Suppression of multiple mouse models of refractory malignancies by reprogramming IL-18 ligand-receptor interaction.
    Article Snippet: Cells were lysed by M-PER Mammalian Protein Extraction Reagent (ThermoScientific).

    Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27.
    Article Snippet: Cells were lysed using M-PERTM Mammalian Protein Extraction Reagent (Thermo Fisher Scientific cat. no. 78501) lysis buffer, supplemented with HaltTM Protease and Phosphatase Inhibitor Single-Use Cocktail (100x) (Thermo Fisher Scientific cat. no. 78442).

    Article Title: Loss of serine/threonine protein phosphatase 6 severely impairs sexual stage development in malaria parasite Plasmodium berghei.
    Article Snippet: Proteins were extracted using M-PER Mammalian Protein Extraction Reagent (Thermo Fisher) and quantified with the Pierce BCA Protein Assay Kit (Thermo Fisher), followed by resolution via 10% SDS-PAGE.

    Article Title: Insulin resistance, cognition, and functional brain network topology in older adults with obesity.
    Article Snippet: Each suspension then received 450ul M-PER mammalian protein extraction reagent (Thermo Fisher Scientific, Incorporated) that had been adjusted to pH 8 with 1 M Tris HCL (pH 8.6) and contained the cocktails of protease and phosphatase inhibitors.

    Article Title: Human induced pluripotent stem cell derived nanovesicles for cardiomyocyte protection and proliferation
    Article Snippet: It remains a significant challenge to reactivate the cell cycle activity of adult mammalian cardiomyocytes (CMs).. This study created a hypo-immunogenic human induced pluripotent stem cell (hiPSC) line using clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 gene editing to knockout β2-microglobulin in hiPSCs (B2MKOhiPSCs) for manufacturing nanovesicles (B2MKOhiPSC-NVs).. Approximately 9500 B2MKOhiPSC-NVs were produced from a single B2MKOhiPSC.

    Article Title: Exogenous pyruvate is therapeutic against colitis by targeting cytosolic phospholipase A2
    Article Snippet: Briefly, RAW264.7 cells were lysed using M-PERTM Mammalian Protein Extraction Reagent (78501, Thermofisher), followed by centrifugation at 18,000 g and 4 °C for 10 min.

    Article Title: Diammonium Glycyrrhizinate Exerts Broad-Spectrum Antiviral Activity Against Human Coronaviruses by Interrupting Spike-Mediated Cellular Entry.
    Article Snippet: Cellular proteins were extracted from cell lysates using M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific, New York, NY, USA) containing halt protease inhibitor single-use cocktail and then denatured in protein loading buffer at 100 ◦C for 10 min. Immunoblotting for HCoV-OC43 nucleocapsid protein (Millipore, Billerica, MA, USA, 1:1000) and HCoV-229E nucleocapsid protein (Sino Biological, Beijing, China, 1:1000) was performed as described previously [40].

    Lysis:

    Article Title: miRNA-29b-1-5p mediates an antiviral activity by targeting the HBV entry receptor in human hepatocytes.
    Article Snippet: Cells were collected 24 h posttransfection, and protein was extracted using the M-PER mammalian protein extraction reagent (ThermoFisher Scientific).

    Article Title: Suppression of multiple mouse models of refractory malignancies by reprogramming IL-18 ligand-receptor interaction.
    Article Snippet: Cells were lysed by M-PER Mammalian Protein Extraction Reagent (ThermoScientific).

    Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27.
    Article Snippet: Cells were lysed using M-PERTM Mammalian Protein Extraction Reagent (Thermo Fisher Scientific cat. no. 78501) lysis buffer, supplemented with HaltTM Protease and Phosphatase Inhibitor Single-Use Cocktail (100x) (Thermo Fisher Scientific cat. no. 78442).

    Article Title: Loss of serine/threonine protein phosphatase 6 severely impairs sexual stage development in malaria parasite Plasmodium berghei.
    Article Snippet: Proteins were extracted using M-PER Mammalian Protein Extraction Reagent (Thermo Fisher) and quantified with the Pierce BCA Protein Assay Kit (Thermo Fisher), followed by resolution via 10% SDS-PAGE.

    Article Title: Insulin resistance, cognition, and functional brain network topology in older adults with obesity.
    Article Snippet: Each suspension then received 450ul M-PER mammalian protein extraction reagent (Thermo Fisher Scientific, Incorporated) that had been adjusted to pH 8 with 1 M Tris HCL (pH 8.6) and contained the cocktails of protease and phosphatase inhibitors.

    Article Title: Human induced pluripotent stem cell derived nanovesicles for cardiomyocyte protection and proliferation
    Article Snippet: It remains a significant challenge to reactivate the cell cycle activity of adult mammalian cardiomyocytes (CMs).. This study created a hypo-immunogenic human induced pluripotent stem cell (hiPSC) line using clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 gene editing to knockout β2-microglobulin in hiPSCs (B2MKOhiPSCs) for manufacturing nanovesicles (B2MKOhiPSC-NVs).. Approximately 9500 B2MKOhiPSC-NVs were produced from a single B2MKOhiPSC.

    Article Title: Exogenous pyruvate is therapeutic against colitis by targeting cytosolic phospholipase A2
    Article Snippet: Briefly, RAW264.7 cells were lysed using M-PERTM Mammalian Protein Extraction Reagent (78501, Thermofisher), followed by centrifugation at 18,000 g and 4 °C for 10 min.

    Article Title: Diammonium Glycyrrhizinate Exerts Broad-Spectrum Antiviral Activity Against Human Coronaviruses by Interrupting Spike-Mediated Cellular Entry.
    Article Snippet: Cellular proteins were extracted from cell lysates using M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific, New York, NY, USA) containing halt protease inhibitor single-use cocktail and then denatured in protein loading buffer at 100 ◦C for 10 min. Immunoblotting for HCoV-OC43 nucleocapsid protein (Millipore, Billerica, MA, USA, 1:1000) and HCoV-229E nucleocapsid protein (Sino Biological, Beijing, China, 1:1000) was performed as described previously [40].

    Suspension:

    Article Title: miRNA-29b-1-5p mediates an antiviral activity by targeting the HBV entry receptor in human hepatocytes.
    Article Snippet: Cells were collected 24 h posttransfection, and protein was extracted using the M-PER mammalian protein extraction reagent (ThermoFisher Scientific).

    Article Title: Suppression of multiple mouse models of refractory malignancies by reprogramming IL-18 ligand-receptor interaction.
    Article Snippet: Cells were lysed by M-PER Mammalian Protein Extraction Reagent (ThermoScientific).

    Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27.
    Article Snippet: Cells were lysed using M-PERTM Mammalian Protein Extraction Reagent (Thermo Fisher Scientific cat. no. 78501) lysis buffer, supplemented with HaltTM Protease and Phosphatase Inhibitor Single-Use Cocktail (100x) (Thermo Fisher Scientific cat. no. 78442).

    Article Title: Loss of serine/threonine protein phosphatase 6 severely impairs sexual stage development in malaria parasite Plasmodium berghei.
    Article Snippet: Proteins were extracted using M-PER Mammalian Protein Extraction Reagent (Thermo Fisher) and quantified with the Pierce BCA Protein Assay Kit (Thermo Fisher), followed by resolution via 10% SDS-PAGE.

    Article Title: Insulin resistance, cognition, and functional brain network topology in older adults with obesity.
    Article Snippet: Each suspension then received 450ul M-PER mammalian protein extraction reagent (Thermo Fisher Scientific, Incorporated) that had been adjusted to pH 8 with 1 M Tris HCL (pH 8.6) and contained the cocktails of protease and phosphatase inhibitors.

    Article Title: Human induced pluripotent stem cell derived nanovesicles for cardiomyocyte protection and proliferation
    Article Snippet: It remains a significant challenge to reactivate the cell cycle activity of adult mammalian cardiomyocytes (CMs).. This study created a hypo-immunogenic human induced pluripotent stem cell (hiPSC) line using clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 gene editing to knockout β2-microglobulin in hiPSCs (B2MKOhiPSCs) for manufacturing nanovesicles (B2MKOhiPSC-NVs).. Approximately 9500 B2MKOhiPSC-NVs were produced from a single B2MKOhiPSC.

    Article Title: Exogenous pyruvate is therapeutic against colitis by targeting cytosolic phospholipase A2
    Article Snippet: Briefly, RAW264.7 cells were lysed using M-PERTM Mammalian Protein Extraction Reagent (78501, Thermofisher), followed by centrifugation at 18,000 g and 4 °C for 10 min.

    Article Title: Diammonium Glycyrrhizinate Exerts Broad-Spectrum Antiviral Activity Against Human Coronaviruses by Interrupting Spike-Mediated Cellular Entry.
    Article Snippet: Cellular proteins were extracted from cell lysates using M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific, New York, NY, USA) containing halt protease inhibitor single-use cocktail and then denatured in protein loading buffer at 100 ◦C for 10 min. Immunoblotting for HCoV-OC43 nucleocapsid protein (Millipore, Billerica, MA, USA, 1:1000) and HCoV-229E nucleocapsid protein (Sino Biological, Beijing, China, 1:1000) was performed as described previously [40].



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